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Stains were quantified by constructing a three-dimensional image using IMARIS, and counted using NEURON TRACER and WinROOF software. Two-way ANOVA followed by a Tukey's post hoc test (P. Then, the number of blue-stained nuclei included in a unit area of 0.25 mm 2 in six randomly selected areas on the upper and lower side of each membranes/meshes was counted under a magnification of ×10, using WinROOF Software (Hirox Co Ltd., Tokyo, Japan). The average number of blue-stained cells from upper side surfaces per total cell number.
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